Platecount is a patented, manual platelet counting solution, dissolving interfering WBC/RBC components while preserving platelets for counting and observation. The platelet counting fluid contains an aggregation inhibitor and a hemolyzing agent. Platecount uses capillary or venous blood with an anticoagulant. This is diluted and mixed in a red cell pipette with diluting fluid. Complete hemolysis of red and white blood cells takes place. You can then count platelets in the hemocytometer.
Directions:
- Fill RBC pipette to 0.5 mark with blood. Fill with Camco Platecount to 101 mark.
- Shake mechanically for not more than one minute.
- Discard 4 to 5 drops from pipette.
- Fill counting chamber and allow to stand 5 to 10 minutes for compete hemolysis of red and white blood cells.
- Count platelets in 10 small squares in finely rule (RBC) area of counting chamber.
Number counted X 5000 = thrombocytes per cubic mm. of blood.
Note: If the count is very low, it is advisable to count more of the finely ruled (RBC) squares and multiple by a smaller factor. For example, count 25 squares and multiple by 2000.
For best results, count platelets as soon as hemolysis is complete.
For In Vitro Diagnostic Use